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酵素名:「EcoR I、bsiW I など」
認識配列:「GAC... など」
オーバーハング:「AC… など」

次のコードが認識配列に表示される場合があります。 N = AまたはCまたはGまたはT(任意)
B = CまたはGまたはT(Aではない)
D = AまたはGまたはT(Cではない)
H = AまたはCまたはT(Gではない)
V = AまたはCまたはG(Tではない)
W = AまたはT(弱い)
S = CまたはG(強い)
R = AまたはG(プリン)
Y = CまたはT(ピリミジン)
M = AまたはC(アミノ))
K = GまたはT(ケト)

横にスクロールできます
酵素名 型番 認識配列 オーバーハング プロパティ イソジゾマー
FG-AatII 3′ ACGT Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available ZraI*
FG-AccI 5′ MK Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available FblI, XmiI
FG-AccIII 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylation Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I, MroI
FG-AcuI 3′ NN Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available Eco57I
FG-AflII 5′ TTAA Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BfrI, BspTI, BstAFI, MspCI, Vha464I
FG-AgeI 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available AsiGI, BshTI, CspAI, PinAI
FG-Alw26I 5′ NNNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available BcoDI, BsmAI, BstMAI
FG-AlwI 5′ N Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available AclWI, BspPI
FG-ApaI 3′ GGCC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available Bsp120I*, PspOMI*
FG-ApaLI 5′ TGCA Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Alw44I, VneI
FG-ApoI 5′ AATT Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available AcsI, XapI
FG-AscI 5′ CGCG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available PalAI, SgsI
FG-AvaI 5′ YCGR Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Ama87I, BmeT110I, BsiHKCI, BsoBI, Eco88I
FG-AvaII 5′ GWC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available Bme18I, Eco47I, SinI, VpaK11BI
FG-AvrII 5′ CTAG Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available AspA2I, BlnI, XmaJI
FG-BalI Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylation MlsI, MluNI, Mox20I, MscI, Msp20I
FG-BamHI 5′ GATC Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available -
FG-BclI 5′ GATC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available FbaI, Ksp22I
FG-BglI 3′ NNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available -
FG-BglII 5′ GATC Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available -
FG-BsaI 5′ NNNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available Bso31I, BspTNI, Eco31I
FG-BsaWI 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available -
FG-BsiWI 5′ GTAC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Pfl23II, PspLI
FG-BsmBI 5′ NNNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Esp3I
FG-BsoBI 5′ YCGR Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available Ama87I, AvaI, BmeT110I, BsiHKCI, Eco88I
FG-BspEI 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available AccIII, Aor13HI, BseAI, Bsp13I, Kpn2I, MroI
FG-BsrFI 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Bse118I, BssAI, Cfr10I
FG-BstYI 5′ GATC Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BstX2I, MflI, PsuI
FG-BtsCI 3′ NN Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BseGI, BstF5I, FokI*
FG-Cfr10I 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Bse118I, BsrFI, BssAI
FG-Cfr42I 3′ GC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available KspI, SacII, Sfr303I, SgrBI
FG-Cfr9I 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylation SmaI*, TspMI, XmaI
FG-ClaI 5′ CG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI
FG-CviAI 5′ GATC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available -
FG-DdeI 5′ TNA Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BstDEI, HpyF3I
FG-DpnI Blunt Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available MalI
FG-DpnII 5′ GATC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available Bsp143I, BssMI, BstKTI*, BstMBI, Kzo9I, MboI, NdeII, Sau3AI
FG-DraI Blunt Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available -
FG-EagI 5′ GGCC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available BseX3I, BstZI, EclXI, Eco52I
FG-Eco47I 5′ GWC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available AvaII, Bme18I, SinI, VpaK11BI
FG-EcoNI 5′ N Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BstENI, XagI
FG-EcoO109I 5′ GNC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available -
FG-EcoRI 5′ AATT Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available -
FG-EcoRV Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Eco32I
FG-EcoT38I 5'GRGC Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BanII, Eco24I, FriOI
FG-Esp3I 5′ NNNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available BsmBI
FG-FokI 5′ NNNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available BseGI*, BstF5I*, BtsCI*
FG-FspI Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Acc16I, NsbI
FG-HaeII 3′ GCGC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available BfoI, BstH2I
FG-HaeIII Blunt Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BshFI, BsnI, BspANI, BsuRI
FG-HgaI 5′ NNNNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available CseI
FG-HincII Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available HindII
FG-HindII Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available HincII
FG-HindIII 5′ AGCT Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available -
FG-HinfI 5′ ANT Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available -
FG-HinP1I 5′ CG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available AspLEI*, BstHHI*, CfoI*, HhaI*, Hin6I, HspAI
FG-HpaI Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available KspAI
FG-HpaII 5′ CG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available BsiSI, HapII, MspI
FG-HphI 3′ N Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available AsuHPI
FG-Hpy188I 3′ N Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available -
FG-Hpy99I 3′ CGWCG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available -
FG-HpyCH4V Blunt Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available HpySE526I, MaeII, TaiI*
FG-Kpn2I 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available AccIII, Aor13HI, BseAI, Bsp13I, BspEI, MroI
FG-KpnI 3′ GTAC Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available Acc65I*, Asp718I*
FG-Lsp1109I 5′ NNNN Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BbvI, BseXI, BstV1I
FG-MboI 5′ GATC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available Bsp143I, BssMI, BstKTI*, BstMBI, DpnII, Kzo9I, NdeII, Sau3AI
FG-MboII 3′ N Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available -
FG-MluI 5′ CGCG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available -
FG-MnlI 3′ N Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available -
FG-MseI 5′ TA Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available SaqAI, Tru1I, Tru9I
FG-MspA1I Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available -
FG-MspI 5′ CG Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BsiSI, HapII, HpaII
FG-MunI 5′ AATT Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available MfeI
FG-NaeI Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available MroNI*, NgoMIV*, PdiI
FG-NcoI 5′ CATG Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available Bsp19I
FG-NdeI 5′ TA Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available FauNDI
FG-NgoMIV 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available MroNI, NaeI*, PdiI*
FG-NheI 5′ CTAG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available AsuNHI, BmtI*, BspOI*
FG-NlaIV Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available BmiI, BspLI, PspN4I
FG-NotI 5′ GGCC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available CciNI
FG-NruI Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available Bsp68I, BtuMI, RruI
FG-NtBstNBI not applicable Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available -
FG-PaeR7I 5′ TCGA Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Sfr274I, SlaI, XhoI
FG-PflMI 3′ NNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylationFast Cut protocol available AccB7I, Van91I
FG-PleI 5′ N Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available MlyI*, PpsI, SchI*
FG-PluTI 3′ GCGC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available DinI*, EgeI*, EheI*, KasI, SfoI*
FG-PspGI 5′ CCWGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to Dam methylation AjnI, BciT130I*, BseBI*, BstNI*, Bst2UI*, EcoRII, MvaI*, Psp6I
FG-PstI 3′ TGCA Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available BspMAI
FG-PvuI 3′ AT Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Ple19I
FG-PvuII Blunt Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available -
FG-RsaI Blunt Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available AfaI, Csp6I*, CviQI*, RsaNI*
FG-SacI 3′ AGCT Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available Psp124BI, SstI, Ecl136II*, EcoICRI*, Eco53kI*
FG-SacII 3′ GC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available Cfr42I, KspI, Sfr303I, SgrBI
FG-SalI 5′ TCGA Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available -
FG-Sau96I 5′ GNC Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available AspS9I, BmgT120I, Cfr13I, PspPI
FG-SbfI 3′ TGCA Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available SdaI, Sse8387I
FG-ScaI Blunt Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available ZrmI
FG-SdaI 3′ TGCA Incubation temperatureSupplied bufferHeat inactivatableFast Cut protocol available SbfI, Sse8387I
FG-SfiI 3′ NNN Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationMay be sensitive to Dam methylationFast Cut protocol available -
FG-SgrAI 5′ CCGG Incubation temperatureSupplied bufferHeat inactivatableMay be sensitive to CpG methylationFast Cut protocol available -

お探しの酵素が見つからない? お問い合わせはこちら

制限酵素とは?

制限酵素は特定の認識部位またはその近くで二本鎖DNAを切断します。
制限酵素は、サブユニット構造、補因子の要件、および切断の特異性に基づいて、4つのタイプに分類されます。
特にタイプⅡの制限酵素は認識配列の部位でDNAを切断するため、実験に非常に役立ちます。
当社でお取り扱いしている制限酵素はすべてこのタイプⅡ由来のものになります。

制限酵素の種類

 

認識配列から離れたランダムな位置でDNAを切断します。これらの酵素は、機能するためにATPとS-アデノシル-L-メチオニンの両方を必要とします。 (※こちらのタイプは取り扱っておりません)

 

認識配列内またはその近くでDNAを切断します。これらの酵素はATPを必要とせず、メチラーゼから独立しています。
タイプIIの酵素は、毎日の実験室作業に最も有用なエンドヌクレアーゼです。
当社でお取り扱いしている制限酵素はすべてこのタイプⅡ由来のものになります。

 

認識配列から約20〜25塩基対離れた位置でDNAを切断します。
これらの酵素は機能するためにATPおよびS-アデノシル-L-メチオニンの両方を必要とします。 (※こちらのタイプは取り扱っておりません)

 

通常メチル化によって阻害されるタイプI〜タイプIIIとは対照的に、修飾された、典型的にはメチル化DNAのみを切断します。(※こちらのタイプは取り扱っておりません)

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